Quantum imaging is a growing field that takes advantage of the counterintuitive
and "spooky" ability of light particles, or photons, to become linked,
or entangled, under specialized circumstances. If the state of one
photon in the entangled duo gets tweaked, so does the other, regardless
of how far apart the two photons might be.
Caltech researchers demonstrated last May
how such entanglement could double the resolution of classical light
microscopes while also preventing an imaging system's light from
damaging fragile biological samples. Now the same team has improved upon
the technique, making it possible to quantum image whole organ slices
and even small organisms.
Led by Lihong Wang, the Bren Professor of Medical Engineering and Electrical
Engineering, the new work uses entanglement—what Albert Einstein once
famously described as "spooky action at a distance"— to control not only
the color and brightness of the light hitting a sample, but also the
polarization of that light.
"Our new technique has the potential to pave the way for quantum imaging in many
different fields, including biomedical imaging and potentially even
remote space sensing," says Wang, who is also the Andrew and Peggy
Cherng Medical Engineering Leadership Chair and executive officer for
medical engineering.
Like wavelength and intensity, polarization is a fundamental property of light and
represents which direction the electric component of a light wave is
oriented with respect to the wave's general direction of travel. Most
light, including sunlight, is unpolarized, meaning that its
electromagnetic waves move and travel in all directions. However,
filters called polarizers can be used to create light beams with one
specific polarization. A vertical polarizer, for example, only allows
photons with vertical polarization to pass through. Those with
horizontal polarization (meaning that the electric component of the
light wave is oriented horizontally with respect to the direction of
travel) will be blocked. Any light with other polarization angles
(between vertical and horizontal), will partially pass through. The
outcome is a stream of vertically polarized light.
This is how polarized sunglasses reduce glare. They use a vertically
polarizing chemical coating to block sunlight that has become
horizontally polarized by reflecting off a horizontal surface, such as a
lake or snowy field. This means that the wearer only observes
vertically polarized light.
When changes in light intensity or color are not enough to give scientists quality
images of certain objects, controlling the polarization of the light in
an imaging system can sometimes provide more information about the
sample and offer a different way to identify contrast between a sample
and its background. Detecting the changes in polarization caused by
certain samples can also give researchers information about the internal
structure and behavior of those materials.
Wang's newest microscopy technique, dubbed quantum imaging by coincidence from
entanglement (ICE), takes advantage of entangled photon pairs to obtain
higher-resolution images of biological materials, including thicker
samples, and to make measurements of materials that have what scientists
call birefringent properties.
Rather than consistently bending incoming light waves in the same way, as most
materials do, birefringent materials bend those waves to different
degrees depending on the light's polarization and the direction in which
it is traveling. The most common birefringent materials studied by
scientists are calcite crystals. But biological materials, such as
cellulose, starch, and many types of animal tissue, including collagen
and cartilage, are also birefringent.
If a sample with birefringent properties is placed between two polarizers
oriented at 90-degree angles to each other, some of the light going
through the sample will be altered in its polarization and will
therefore make it through to the detector, even though all the other
incoming light should be blocked by the two polarizers. The detected
light can then provide information about the structure of the sample. In
materials science, for example, scientists use birefringence
measurements to get a better understanding of the areas where mechanical
stress builds up in plastics.
In Wang's ICE setup, light is passed first through a polarizer and then through a
pair of special barium borate crystals, which will occasionally create
an entangled photon pair; about one pair is produced for every million
photons that pass through the crystals. From there, the two entangled
photons will branch off and follow one of the system's two arms: one
will travel straight ahead, following what is called the idler arm,
while the other traces a more circuitous path called the signal arm that
causes the photon to pass through the object of interest. Finally, both
photons go through an additional polarizer before reaching two
detectors, which record the time of arrival of the detected photons.
Here, though, occurs a "spooky" quantum effect because of the entangled
nature of the photons: the detector in the idler arm can act as a
virtual "pinhole" and "polarization selector" on the signal arm,
instantly affecting the location and polarization of the photon incident
on the object in the signal arm.
"In the ICE setup, the detectors in the signal and idler arms function as 'real'
and 'virtual' pinholes, respectively," says Yide Zhang, lead author of
the new paper and a postdoctoral scholar fellowship trainee in medical
engineering at Caltech. "This dual pinhole configuration enhances the
spatial resolution of the object imaged in the signal arm. Consequently,
ICE achieves higher spatial resolution than conventional imaging that
utilizes a single pinhole in the signal arm."
"Since each entangled photon pair always arrives at the detectors at the same
time, we can suppress noises in the image caused by random photons,"
adds Xin Tong, co-author of the study and a graduate student in medical
and electrical engineering at Caltech.
To determine the birefringent properties of a material with a classical
microscopy setup, scientists typically switch through different input
states, illuminating an object separately with horizontally, vertically,
and diagonally polarized light, and then measuring the corresponding
output states with a detector. The goal is to measure how the
birefringence of the sample alters the image that the detector receives
in each of those states. This information informs scientists about the
structure of the sample and can provide images that would not otherwise
be possible.
Since quantum entanglement allows paired photons to be linked no matter how far apart they might
be, Wang is already imagining how his new system could be used to make
birefringence measurements in space. Consider a situation where
something of interest, perhaps an interstellar medium, is located light
years away from Earth. A satellite in space might be positioned such
that it could emit entangled photon pairs using the ICE technique, with
two ground stations acting as detectors. The large distance to the
satellite would make it impractical to send any kind of signal to adjust
the device's source polarization. However, due to entanglement,
changing the polarization state in the idler arm would be equivalent to
changing the polarization of the source light before the beam hits the
object. "Using quantum technology, nearly instantaneously, we can make
changes to the polarization state of the photons no matter where they
are," Wang says. "Quantum technologies are the future. Out of scientific
curiosity, we need to explore this direction."
A paper describing the work,
"Quantum imaging of biological organisms through spatial and
polarization entanglement," appears in the March 8 issue of the journal
Science Advances.
In addition to Wang, Zhang, and Tong, the paper's co-authors are
medical engineering graduate student David Garrett, postdoctoral scholar
research associate Rui Cao, and former postdoctoral scholar research
associate Zhe He, who is now at the Shandong Institute of Advanced
Technology. The work was supported by funding from Caltech's Center for
Sensing to Intelligence and the National Institutes of Health.